Skip to main content
SpringerLink
Log in

Molecular and biological characterization of Chrysanthemum stem necrosis virus isolates from distinct regions in Japan

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Three isolates of Chrysanthemum stem necrosis virus (CSNV) were obtained from chrysanthemum plants in distinct regions of Japan in 2006 and 2007. All the original host plants showed severe necrotic symptoms on the leaves and stems. Amino acid sequence data of the nucleocapsid protein genes of the three isolates (CbCh07A, TcCh07A, and GnCh07S) showed high identities with those of two other CSNV isolates, HiCh06A L1 from Japan and Chry1 from Brazil. Furthermore, for the first time the complete nucleotide sequence of the S RNA was determined for CSNV (isolate HiCh06A). In phylogenetic analysis based on the non-structural protein genes from the genus Tospovirus, HiCh06A L1 was placed in the same genetic group as Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus. Host range examination for isolates HiCh06A L1 and CbCh07A showed that green pepper (cv. ‘Kyoyutaka’, ‘Saitamawase’, ‘Tosakatsura’, ‘L3 sarara’ and ‘L3 miogi’) and tomato (cv. ‘Sekaiichitomato’) were systemically susceptible hosts, whereas TSWV-resistant Solanaceae species, Capsicum chinense, Lycopersicon peruvianum and a TSWV-resistant cultivar of green pepper (cv. TSR miogi), were resistant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Bezerra, I. C., Resende, R. D. O., Pozzer, L., Nagata, T., Kormelink, R., & De Ávila, A. C. (1999). Increase of tospoviral diversity in Brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. Phytopathology, 89, 823–830.

    Article  PubMed  CAS  Google Scholar 

  • Boiteux, L. S. (1995). Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense. Theoretical and Applied Genetics, 90, 146–149.

    Article  Google Scholar 

  • Bucher, E., Sijen, T., De Haan, P., Goldbach, R., & Prins, M. (2003). Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. Journal of Virology, 77, 1329–1336.

    Article  PubMed  CAS  Google Scholar 

  • De Haan, P., Wagemakers, L., Peters, D., & Goldbach, R. (1990). The S RNA segment of tomato spotted wilt virus has an ambisense character. The Journal of General Virology, 71, 1001–1007.

    Article  PubMed  Google Scholar 

  • Duarte, L. M. L., Rivas, E. B., Alexandre, M. A. V., Chagas, C. M., & Pitta, G. P. B. (1993). Identificação de um isolado severo do vírus do viracabeça do tomateiro em crisântemos. (Abstr.). Fitopatologia Brasileira, 18, 292–293.

    Google Scholar 

  • Duarte, L. M. L., Rivas, E. B., Alexangre, M. A. V., De Ávila, A. C., Nagata, T., & Chagas, C. M. (1995). Chrysanthemum stem necrosis caused by a possible novel tospovirus. Journal of Phytopathology, 143, 569–571.

    Article  Google Scholar 

  • German, T. L., Ullman, D. E., & Moyer, J. W. (1992). Tospoviruses, diagnosis, molecular biology, phylogeny, and vector relationships. Annual Review of Phytopathology, 30, 315–348.

    Article  PubMed  CAS  Google Scholar 

  • Guindon, S., & Gascuel, O. (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696–704.

    Article  PubMed  Google Scholar 

  • Hassani-Mehraban, A., Botermans, M., Verhoeven, J. Th. J., Meekes, E., Saaijer, J., Goldbach, R., et al. (2010). A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Columbia. Archives of Virology, 155, 423–428.

    Article  PubMed  CAS  Google Scholar 

  • Matsuura, S., Kubota, K., & Okuda, M. (2007). First report of Chrysanthemum stem necrosis virus on chrysanthemum in Japan. Plant Disease, 91, 468.

    Article  Google Scholar 

  • Mumford, R. A., Jarvis, B., Morris, J., & Blockley, A. (2003). First report of Chrysanthemum stem necrosis virus (CSNV) in the UK. Plant Pathology, 52, 779.

    Article  Google Scholar 

  • Nagata, T., & De Ávila, A. C. (2000). Transmission of chrysanthemum stem necrosis virus, a recently discovered tospovirus, by two thrips species. Journal of Phytopathology, 148, 123–125.

    Article  Google Scholar 

  • Nagata, T., De Melo, P. C. T., Barbosa, C. J., Juliatti, J. C., Kitajima, E. W., & De Ávila, A. C. (1995). Occurrence of different tospoviruses in six states of Brazil. Fitopatologia Brasileira, 20, 90–95.

    Google Scholar 

  • Nagata, T., Resende, R. O., Kitajima, E. W., Costa, H., Inoue-Nagata, A. K., & De Ávila, A. C. (1998). First report of natural occurrence of zucchini lethal chlorosis tospovirus on cucumber and chrysanthemum stem necrosis tospovirus on tomato in Brazil. Plant Disease, 82, 1403.

    Article  Google Scholar 

  • Nagata, T., Carvalho, K. R., Sodré, R. D. A., Dutra, L. S., Oliveira, P. A., Noronha, E. F., et al. (2007). The glycoprotein gene of Chrysanthemum stem necrosis virus and Zucchini lethal chlorosis virus and molecular relationship with other tospoviruses. Virus Genes, 35, 785–79.

    Article  PubMed  CAS  Google Scholar 

  • Nichol, S. T., Beaty, B. J., Elliott, R. M., Goldbach, R., Plyusnin, A., Schmaljohn, C. S., et al. (2005). Bunyaviridae. In C. M. van Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, & L. A. Ball (Eds.), Virus taxonomy. Eighth report of the international committee on taxonomy of viruses (pp. 695–716). San Diego: Academic Press.

    Google Scholar 

  • Okuda, M., & Hanada, K. (2001). RT-PCR for detecting five distinct Tospovirus species using degenerate primers and dsRNA template. Journal of Virological Methods, 96, 149–156.

    Article  PubMed  CAS  Google Scholar 

  • Ravnikar, M., Vozelj, N., Mavrie, I., Svigelj, S. D., Zupaneie, M., & Petrovie, N. (2003). Detection of Chrysanthemum stem necrosis virus and Tomato spotted wilt virus in chrysanthemum. Abstracts 8th International Congress of Plant Pathology ICPP, Christchurch (NZ).

  • Rosello, S., Soler, S., Diez, M. J., Rambla, J. L., Richarte, C., & Nuez, F. (1999). New sources for high resistance of tomato to the tomato spotted wilt virus from Lycopersicon peruvianum. Plant Breeding, 118, 425–429.

    Article  Google Scholar 

  • Takeda, A., Sugiyama, K., Nagano, H., Mori, M., Kaido, M., Mise, K., et al. (2002). Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Letters, 532, 75–79.

    Article  PubMed  CAS  Google Scholar 

  • Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., & Higgins, D. G. (1997). The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24, 4876–4882.

    Article  Google Scholar 

  • Verhoeven, J. Th. J., Roenhorst, J. W., Cortes, I., & Peters, D. (1996). Detection of a novel tospovirus in chrysanthemum. Acta Horticulturae, 432, 44–51.

    Google Scholar 

Download references

Acknowledgement

Authors would like to thank Mr. Shinichiro Okazaki, Oita Prefectural Agriculture, Forestry and Fisheries Research Center, for providing F. occidentalis and for the helpful suggestions with regard to the transmission tests, and Mr. Osamu Nunomura, Japan Horticultural Production and Research Institute, Division of extension for production, for providing ‘TSR miogi’. Germplasm of Capsicum chinense and Lycopersicon peruvianum was provided by National Institute of Agrobiological Sciences, Japan.

Author information

Authors and Affiliations

  1. Laboratory of Plant Pathology, Kyushu University, 6-10-1 Hakozaki Higashi-ku, Fukuoka, 812–8581, Japan

    Minoru Takeshita, Naoko Nagai, Naruto Furuya & Kenichi Tsuchiya

  2. National Agricultural Research Center for Kyushu Okinawa Region, Kikuchi, Kumamoto, 861–1192, Japan

    Mitsuru Okuda & Shiori Okuda

  3. Hiroshima Prefectural Technology Research Institute, Agriculture Research Center, Higashi-hiroshima, Hiroshima, 739–0151, Japan

    Shohei Matsuura

Authors
  1. Minoru Takeshita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naoko Nagai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mitsuru Okuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shohei Matsuura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shiori Okuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Naruto Furuya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kenichi Tsuchiya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Minoru Takeshita.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takeshita, M., Nagai, N., Okuda, M. et al. Molecular and biological characterization of Chrysanthemum stem necrosis virus isolates from distinct regions in Japan. Eur J Plant Pathol 131, 9–14 (2011). https://doi.org/10.1007/s10658-011-9797-z

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-011-9797-z

Keywords

Search

Navigation